Liquid nitrogen biological container was manufactured according to the theory of vacuum interlayer insulation founded by a British scientist named Dewar in 1898,which solved the problem of mass evaporation of liquid nitrogen stored because of heat convection, conduction and radiation. With the progress of the society and the development of the technology, the technology of high vacuum multilayer insulation, new materials and other new technologies are applied ,which makes liquid nitrogen biological container have excellent performance of thermal insulation and can minimize the loss of liquid nitrogen(－196℃) stored. Therefore, liquid nitrogen biological container is widely used with liquid nitrogen as its refrigerant in the fields of animal husbandry, medical treatment, scientific research and machine processing for storing semen, vaccine and other biological sample, cold processing of metal materials and cold assembling of precise apparatus.